DuPont BAX System AOAC-RI Validation Extended for Faster Listeria Detection

The AOAC Research Institute (AOAC-RI), a subsidiary of AOAC International in Rockville, Md., has granted a method extension for the DuPont BAX System real-time PCR assays for Listeria to include validation of a new proprietary enrichment media. This method can return Listeria results in 22 hours for some matrices.

The AOAC-RI method allows customers to perform a single-stage, 20-26 hour enrichment (sample-dependent) in FoodChek Actero Listeria Enrichment Media, followed by rapid real-time processing with the DuPont BAX System. The extension includes detection of both Listeria species (AOAC-RI Performance Tested Method #081401) and L. monocytogenes (AOAC-RI Performance Tested Method #121402) and has been tested in frankfurters, shrimp, smoked salmon, spinach and queso fresco, as well as on stainless steel, plastic and sealed concrete surfaces.

“Enriching food samples in Actero™ Listeria Enrichment media allows laboratories to use about 33 percent less media per sample than other methods,” said FoodChek Chairman, President and CEO William Hogan. “This not only results in less waste disposal during the testing process, but also helps improve the financial bottom line for food companies and commercial testing laboratories alike.”

The DuPont BAX System provides automated testing for foodborne pathogens, spoilage organisms and other microbes in raw ingredients, finished products, and manufacturing environments. The system breaks down samples at the genetic level using the power of the polymerase chain reaction (PCR), then uses target-specific primers to automatically detect the presence or absence of the target bacterial DNA and provide clear yes-or-no results