Bio-Rad Laboratories announced that AOAC International has evaluated and approved the level 3 modification of the iQ-Check E. coli O157:H7 and iQ-Check STEC VirX and SerO real-time PCR detection kits to extend these kits to different types of samples. AOAC is a globally recognized third-party association that develops microbiological and chemical standards to facilitate public health and safety.
To obtain this approval through the AOAC Performance Tested Methods Program, a validation study was performed that demonstrated no differences between the iQ-Check methods and the reference methods. The iQ-Check E. coli O157:H7 assay was modified to include 375-gram test portions of raw ground beef (83% lean), raw beef trim, and fresh spinach. It was also modified to include 25-gram test portions of raw chicken breast without skin, raw chicken thigh with skin, mechanically separated chicken and raw ground pork.
The iQ-Check STEC VirX and SerO assays were modified to include 375-gram test portions of raw ground beef, raw beef trim and fresh spinach from buffered peptone water.
The level 3 modification also included the use of the iQ-Check Free DNA Removal Solution and a single enrichment in buffered peptone water. This harmonized method also allows for the simultaneous enrichment and subsequent detection of Salmonella and pathogenic E. coli from the same sample, providing both a time and cost savings when testing large sample sizes.
The iQ-Check kits for foodborne pathogen detection are routinely used in food safety programs worldwide and are recognized by renowned international validation organizations. For more information on Bio-Rad’s iQ-Check real-time PCR test kits, visit http://www.bio-rad.com/iqcheck.